RHODIOLA ROSEA AND FERULIC ACID ACTIVATE EXPRESSION OF GENES RELATED TO AUTOPHAGY AND RESISTANCE TO HEAT SHOCK IN MICE OF DIFFERENT AGE

The aim of our study was testing whether R. rosea extract and ferulic acid activate expression targets FoxO, regulators energy metabolism autophagy in livers young old mice, to what extent the effects on genes studied coincide. Methods. . C57BL/6J mice were reared at 22 ± 2 °С, 50-60% humidity, 12/12 hour light/dark cycle. All groups a standard chow (4,8% fats, 21,8% protein, 3,9% fibre). Experimental consumed water, supplemented with either sodium ferulate or during 12 weeks prior sacrificing. amounts adjusted provide 4 mg phenol-containing substances per 100 g weight, for mouse, 24 hours. We tested three-month-old (“young”) twelve-month-old males (“old”). levels messenger ribonucleic (mRNA) assessed using AriaMx real-time polymerase chain reaction (RT-PCR) instrument (Agilent). Ribonucleic purified Monarch Miniprep kit (New England BioLabs (NEB), T2010), complementary deoxyribonucleic synthesis performed ProtoScript II (NEB, E6560), quantitative RT-PCR (qRT-PCR) Luna Universal E3003). ATG5 (an marker), HSPB8 (a small heat shock an FoxO target), UCP2 (uncoupling protein 2, senescence CDKN2 (cell cycle regulator, PDK2 PDK4 (pyruvate dehydrogenase kinases 4, oxidative metabolism), TFEB (transcription factor EB, transcriptional regulator autophagy) evaluated. Results. Livers that food had 3.2-fold 3.6-fold higher mRNA HspB8 than control respectively. In this 3.3-fold diet containing as compared control. However, there no significant difference between those ferulate-supplemented food. induced by 4.3 6.6 times from level, Ferulate also affected mice. 1.5-fold Conclusions. Both, one its active components – promote increasing PDK4, coding pyruvate kinase HSPB8, ATG5, PDK2, PDK4. Thus, exert similar gene supposed activation response autophagy, concomitant inhibition mitochondrial via boosting